World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Genetics : Pps, a Large Multidomain Protein, Functions with Sex- Lethal to Regulate Alternative Splicing in Drosophila, Volume 6

By Stubbs, Lisa

Click here to view

Book Id: WPLBN0003929814
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Genetics : Pps, a Large Multidomain Protein, Functions with Sex- Lethal to Regulate Alternative Splicing in Drosophila, Volume 6  
Author: Stubbs, Lisa
Volume: Volume 6
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection, PLoS Genetics
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Stubbs, L. (n.d.). Plos Genetics : Pps, a Large Multidomain Protein, Functions with Sex- Lethal to Regulate Alternative Splicing in Drosophila, Volume 6. Retrieved from http://members.worldlibrary.net/


Description
Description : Alternative splicing controls the expression of many genes, including the Drosophila sex determination gene Sex-lethal (Sxl). Sxl expression is controlled via a negative regulatory mechanism where inclusion of the translation-terminating male exon is blocked in females. Previous studies have shown that the mechanism leading to exon skipping is autoregulatory and requires the SXL protein to antagonize exon inclusion by interacting with core spliceosomal proteins, including the U1 snRNP protein Sans-fille (SNF). In studies begun by screening for proteins that interact with SNF, we identified PPS, a previously uncharacterized protein, as a novel component of the machinery required for Sxl male exon skipping. PPS encodes a large protein with four signature motifs, PHD, BRK, TFS2M, and SPOC, typically found in proteins involved in transcription. We demonstrate that PPS has a direct role in Sxl male exon skipping by showing first that loss of function mutations have phenotypes indicative of Sxl misregulation and second that the PPS protein forms a complex with SXL and the unspliced Sxl RNA. In addition, we mapped the recruitment of PPS, SXL, and SNF along the Sxl gene using chromatin immunoprecipitation (ChIP), which revealed that, like many other splicing factors, these proteins bind their RNA targets while in close proximity to the DNA. Interestingly, while SNF and SXL are specifically recruited to their predicted binding sites, PPS has a distinct pattern of accumulation along the Sxl gene, associating with a region that includes, but is not limited to, the SxlPm promoter. Together, these data indicate that PPS is different from other splicing factors involved in male-exon skipping and suggest, for the first time, a functional link between transcription and SXL–mediated alternative splicing. Loss of zygotic PPS function, however, is lethal to both sexes, indicating that its role may be of broad significance.

 

Click To View

Additional Books


  • Plos Genetics : Genome-wide Association ... (by )
  • Plos Genetics : H-ns Can Facilitate Spec... (by )
  • Plos Genetics : Breakpoint Analysis of T... (by )
  • Plos Genetics : a Germline Polymorphism ... (by )
  • Plos Genetics : Dna Methylation Restrict... (by )
  • Plos Genetics : a Population Genetics-ph... (by )
  • Plos Genetics : Differential Gene Expres... (by )
  • Plos Genetics : Multiple Roles and Inter... (by )
  • Plos Genetics : Genetic Association Stud... (by )
  • Plos Genetics : Molecular Mechanisms Gen... (by )
  • Plos Genetics : the Transcriptomes of Tw... (by )
  • Plos Genetics : an Evolutionarily Conser... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.