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Plos Genetics : Role of Individual Subunits of the Neurospora Crassa Csn Complex in Regulation of Deneddylation and Stability of Cullin Proteins, Volume 6

By Deng, Xing Wang

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Book Id: WPLBN0003929836
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Reproduction Date: 2015

Title: Plos Genetics : Role of Individual Subunits of the Neurospora Crassa Csn Complex in Regulation of Deneddylation and Stability of Cullin Proteins, Volume 6  
Author: Deng, Xing Wang
Volume: Volume 6
Language: English
Subject: Journals, Science, Genetics
Collections: Periodicals: Journal and Magazine Collection, PLoS Genetics
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Publisher: Plos

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Deng, X. W. (n.d.). Plos Genetics : Role of Individual Subunits of the Neurospora Crassa Csn Complex in Regulation of Deneddylation and Stability of Cullin Proteins, Volume 6. Retrieved from http://members.worldlibrary.net/


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Description : The Cop9 signalosome (CSN) is an evolutionarily conserved multifunctional complex that controls ubiquitin-dependent protein degradation in eukaryotes. We found seven CSN subunits in Neurospora crassa in a previous study, but only one subunit, CSN-2, was functionally characterized. In this study, we created knockout mutants for the remaining individual CSN subunits in N. crassa. By phenotypic observation, we found that loss of CSN-1, CSN-2, CSN-4, CSN-5, CSN-6, or CSN-7 resulted in severe defects in growth, conidiation, and circadian rhythm: the defect severity was gene-dependent. Unexpectedly, CSN- 3 knockout mutants displayed the same phenotype as wild-type N. crassa. Consistent with these phenotypic observations, deneddylation of cullin proteins in csn-1, csn-2, csn-4, csn-5, csn-6, or csn-7 mutants was dramatically impaired, while deletion of csn-3 did not cause any alteration in the neddylation/deneddylation state of cullins. We further demonstrated that CSN-1, CSN-2, CSN-4, CSN-5, CSN-6, and CSN-7, but not CSN-3, were essential for maintaining the stability of Cul1 in SCF complexes and Cul3 and BTB proteins in Cul3-BTB E3s, while five of the CSN subunits, but not CSN-3 and CSN-5, were also required for maintaining the stability of SKP-1 in SCF complexes. All seven CSN subunits were necessary for maintaining the stability of Cul4-DDB1 complexes. In addition, CSN-3 was also required for maintaining the stability of the CSN-2 subunit and FWD-1 in the SCFFWD-1 complex. Together, these results not only provide functional insights into the different roles of individual subunits in the CSN complex, but also establish a functional framework for understanding the multiple functions of the CSN complex in biological processes.

 

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